Lycobetaine acetate (Oxylycorine) is a potential biofungicide against Penicillium roqueforti and Aspergillus niger.2. Ungeremine have strong antibacterial activity against Flavobacterium columnare

Lycobetaine针对包括药敏感型和多重药物耐药（MDR）表型在内的9种癌症细胞线展示了细胞毒性活性。其半抑制浓度（IC50）值变化范围为3.67 μM（作用于MDA-MB-231-BCRP乳腺癌细胞）至75.24 μM（针对CEM/ADR5000白血病细胞）。与之相比，用作对照化合物的多柔比星（doxorubicin）的IC50值从0.02 μM（作用于CCRF-CEM细胞）到122.96 μM（针对CEM/ADR5000细胞）。Lycobetaine还能诱导铁死亡、坏死性细胞死亡、自噬及通过激活半胱天冬酶、改变线粒体膜电位（MMP）及增加活性氧（ROS）产生介导的凋亡[1]。

The cytotoxicity, ferroptotic and necroptotic cell death were determined by the resazurin reduction assay. Caspase activation was evaluated using the caspase-Glo assay. Flow cytometry was applied for the analysis of cell cycle analysis (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP) (JC-1) and reactive oxygen species (ROS) (H2DCFH-DA). Apoptotic, necroptotic and autophagic markers were determined by Western blotting. CCRF-CEM leukemia cells were used for all mechanistic studies[1].